Estimation of Population Size and Dispersal Pattern of Sterile Male Aedes aegypti Using Mark-Release-Recapture (MRR) Technique

R. Zulfa, S. Yuliawati, M. Martini, R. Hestiningsih, B. Ernawan


Aedes aegypti is currently emerging as a main vector of Dengue, Zika, and Chikungunya transmission. Chemical control was reported to be less effective due to the resistance of this mosquito to some types of insecticides. Therefore, another vector control is needed which is most appropriate to be used, i.e. the sterile insect technique (SIT). Information about optimum range dispersal sterile male Aedes aegypti for optimalization SIT program are needed. This study was designed to determine the dispersal pattern and population estimation of Aedes aegypti sterilized with gamma rays using mark-release-recapture (MRR) method. After the male Aedes aegypti (pupal stage) was irradiated with 70 Gy of gamma rays, the mosquitoes were then marked with Rhodamine-B and released into the study site. MRR experiments were carried out in Batan Indah residential area, and the Aedes aegypti were released in center of the site. Mosquitoes were recaptured at 28 points spread over the Batan Indah Residence for 2, 4, 6, and 8 days after release by using BG-Sentinel Traps. The result showed that the population of Aedes aegypti in the site was estimated to be 5.402 (1.347–14.636; CI 95 %) with the furthest spread distance was 119 meters from the release point. This study also showed that the MRR experiment can be used to estimate the population size and dispersal pattern of Aedes aegypti movement in a given locality. The result of present study provide better understanding for optimalization mosquito-borne disease prevention based on SIT programs.


Sterile Insect Technique (SIT); Rhodamine-B; Ae. aegypti; Mark-Release-Recapture (MRR)

Full Text:



Anonymous, Vector-Borne Diseases. detail/vector-bornediseases. Retrieved in July (2020).

Anonymous, CDC, Surveillance and Control of Aedes aegypti and Aedes albopictus, in the United States. surveillance-and-control-of-aedes-aegypti-andaedes-albopictus-us.pdf. Retrieved in August (2020)

C. L. Moyes, J. Vontas, A. J. Martins et al., PLoS Negl. Trop. Dis. 11 (2017) 1.

Anonymous, Evaluation of innovative strategies for Aedes aegypti control: challenges for their introduction and impact assessment, Pan American Health Organization, Washington, D.C (2019).

Anonymous, Guidance framework for testing the sterile insect technique as a vector control tool against Aedes-borne diseases, World Health Organization and the International Atomic Energy Agency, Geneva, Switzerland (2020).

P. Kittayapong, S. Ninphanomchai, W. Limophasmanee et al., Plos Negl. Trop. Dis. 13 (2019) 1.

J. W. Mains, P. H. Kelly, K. L. Dobson et al., J. Med. Entomol. 56 (2019) 1296.

J. Bouyer, H. Yamada, R. Pereira et al., Trends Parasitol. 36 (2020) 325.

J. E. Crawford, D. W. Clarke, V. Criswell et al., Nat. Biotechnol. 38 (2020) 482.

H. A. Flores, S. L. O’Neill, Nat. Rev. Microbiol. 16 (2018) 508.

D. Sutiningsih, A. Rahayu and D. Puspitasari,. J. Trop. Life Sci. 7 (2017) 133.

M. Q. Benedict, J. D. Charlwood, L. C. Harrington et al., Vector-Borne Zoonotic Dis. 18 (2018) 39.

B. J. Johnson, S. N. Mitchell, C. J. Paton et al., PLoS Negl. Trop. Dis. 11 (2017) e0005902.

C. A. Guerra, R. C. Reiner, T. A. Perkins et al., Parasites Vectors 7 (2014) 1.

M. C. Medeiros, E. C. Boothe, E. B. Roark et al., PLoS Negl. Trop. Dis. 11 (2017) e0005347.

M. A. Opiyo, G. L. Hamer, D. W. Lwetoijera et al., PLoS One 11 (2016) 0159067.

Anonymous, South Tangerang in Figures, Statistic Department of South Tangerang City (2017).

B. Ernawan, U. S. F. Tambunan, I. Sugoro et al., Effects of Gamma Irradiation Dose-Rate on Sterile Male Aedesaegypti, Proceeding of International Biology Conference (2016).

W. K. Reisen, F. Mahmood and T. Parveen, Popul. Ecol. 21 (1979) 12.

N. T. J. Bailey, J. Anim. Ecol. 21 (1952) 120.

M. Niera, R. Lacroix, L. Caceres et al., Mem. Inst. Oswaldo Cruz. 109 (2014) 879.

W. Tantowijoyo, E. Arguni, P. Johnson et at., J. Med. Entomol. 53 (2016) 188.

V. J. Matthew, C. Brenda, R. J. Gregory et al., Parasites Vectors 13 (2020) 543.

L. Valerio, L. Facchinelli, J. M. Ramsey et al., Am. J. Trop. Med. Hyg. 86 (2012) 665.

J. G. Juarez, G. L. Selene, L. F. Chaves et al., Sci. Rep. 10 (2020) 6803.

M. Martini, A. Zufri, N. Kusariana et al., Entomological Status Based on Vector Density Index and Transovarial Infection on Aedes Sp. Mosquito in Meteseh Village, Semarang City, E3S Web of Conferences 202 (2020) 12016.


Copyright (c) 2020 Atom Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.