Synthesis of Polyvinyl Alcohol (PVA)-Gelatin Hydrogel from White Snapper (Lates calcarifer, Bloch) with Gamma Irradiation and Its Characterizations

H Hariyanti, E Erizal, R. Z. Apriyani, D. P. Perkasa, I. Lestari, H. Rahmi


The application of nuclear technology in the health sector is increasing. One example is the use of irradiation in production of wound dressings. Research activities have been conducted to study whether polyvinyl alcohol (PVA)-gelatin-based hydrogel from white snapper scales can be processed using gamma irradiation into wound dressings. A series of PVA (10 %) solutions containing gelatin in various concentrations (0-4 %) were treated with three freeze-thaw cycles and then irradiated at doses of 10 and 20 kGy. They were subsequently characterized using Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). Gel fraction, water absorption, and percentage of hydrogel water evaporation rate were tested gravimetrically, while the elongation at break and the tensile strength of the hydrogels were tested with a universal testing meter. The evaluation showed that the hydrogel gel fraction decreased with increasing gelatin concentration from 0 % to 4 % for both irradiation doses (10 and 20 kGy). The rising gelatin concentration demonstrated that increasing gamma radiation dose improved the hydrogel's water absorption, evaporation rate, tensile strength, and elongation at break. PVA-gelatin hydrogel with irregular pore structure was observed from SEM test results. The FTIR measurement results confirmed the formation of crosslinks in the hydrogel matrix. The PVA-gelatin hydrogel produced through gamma irradiation could be used for wound dressings.


Polyvinyl alcohol (PVA); Gelatin; Gamma irradiation; Hydrogel; Wound dressing

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D. Darwis and B. Abbas. Aplikasi Isotop dan Radiasi dalam Pembuatan dan Pengembangan Bahan Biomaterial untuk Keperluan Klinis. Prosiding Seminar Nasional Keselamatan Kesehatan dan Lingkungan VI (2010) 60. (in Indonesian).

J. Alipal, N. A. S. M. Pu’ad, T. C. Lee et al., A Review of Gelatin: Properties, Sources, Process, Applications and Commercialisation, Materials Today: Proceedings 42 (2021) 240.

A. M. Hameed, T. Asiyanbi-H, M. Idris et al., Trop. Life Sci. Res. 29 (2018) 213.

Z. A. N. Hanani, Y. H. Roos and J. P. Kerry, Food Hydrocolloids 29 (2012) 144.

Dian P. P., Darmawan, Erizal et al., Jurnal Sains Materi Indones. 14 (2012) 40. (in Indonesian).

Erizal and Z. Abidin, Jurnal Ilmiah Aplikasi Isotop dan Radiasi 7 (2011) 21. (in Indonesian).

S. Sinthusamran, S. Benjakul and H. Kishimura, Food Chem. 152 (2014) 276.

B. G. Bhernama, R. S. Nasution and S. U. Nisa, Jurnal Sains Natural 10 (2020) 43. (in Indonesian).

W. Sukhlaaied and S. A. Riyajan, J. Polym. Environ. 22 (2014) 350.

S. Riyajan, W. Sukhlaaied and W. Keawmang, Carbohydr. Polym. 122 (2015) 301.

Hariyanti, Erizal, M. Y. Yunus et al., Jurnal Sains Materi Indonesia 21 (2020) 143.

(in Indonesian).

S. Tavakoli and A. S. Klar, Biomol. 10 (2020) 1169.

S. P. Ndlovu, K. Ngece, S. Alven et al., Polym. 13 (2021) 2959.

Erizal, D. Pribadi, E. Yulianti et al., Jurnal Sains Materi Indonesia 19 (2018) 139.

(in Indonesian).

Hariyanti, Erizal, E. Mustikarani et al., Atom Indones. 48 (2022) 37.

A. T. Naikwadi, B. K. Sharma, K. D. Bhatt et al., Front. Chem. 10 (2022) 837111.

Erizal, B. Abbas, Sudirman et al., Jurnal Kimia Kemasan 34 (2012) 192. (in Indonesian).

Q. Xing, K. Yates, C. Vogt et al., Sci. Rep. 4 (2014) 1.

Erizal, W. Pratiwi, D. P. Perkasa et al., Jurnal Kimia Kemasan 40 (2018) 47. (in Indonesian).

E. M. Ahmed, J. Adv. Res. 6 (2015) 105.

K. Nie, S. Han, J. Yang et al., Polym. 12 (2020) 1977.

Y. Dong, S. Zhao, W. Lu et al., RSC Adv. 11 (2021) 10794.

A. Saarai, V. Kasparkova, T. Sedlacek et al., A Comparative Study of Crosslinked Sodium Alginate/Gelatin Hydrogels for Wound Dressing. Proceedings of the 5th WSEAS International Conference on Energy and Development – Environment - Biomedicine (EDEB’11) (2011) 384.

N. C. Homem, T. D. Tavares, C. S. Miranda et al., Int. J. Mol. Sci. 22 (2021) 1930.

Erizal, Tjahjono, Dian P. P., Darmawan, Indones. J. Chem. 13 (2013) 41.

X. Han, M. Li, Z. Fan et al., Macromol. Chem. Phys. 221 (2020) 2000237.

T. Miao, E. J. Miller, C. McKenzie et al., J. Mater. Chem. B 3 (2015) 9242.

T. Miao, S. L. Fenn, P. N. Charron et al., Biomacromolecules 16 (2015) 3740.


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