Application of Radiolabeled Technique in Indonesia: A Receptor Binding Assay (RBA) Approach for Analysing Paralytic Shellfish Poisoning (PSP) Toxins in Green Mussels from Lampung Bay

U. Sugiharto, E. Rohaeti, H. Purwaningsih, A. A. Lubis, T. R. D. Larasati, D. Shintianata, F. P. Andini, S. S. Meiliastri, M Muawanah

Abstract


The Receptor Binding Assay (RBA), using radiolabeled [³H]-saxitoxin as a tracer, was applied to detect Paralytic Shellfish Poisoning (PSP) toxins in green mussels (Perna viridis) from Lampung Bay, Indonesia. Sampling was conducted during Harmful Algal Bloom (HAB) events in 2012, 2014, and 2015, which coincided with mass fish mortality linked to red tide phenomena. The RBA achieved a markedly lower detection limit (0.2 µg STX eq. /100 g) than the conventional Mouse Bio-Assay (MBA) of 40 µg STX eq. /100 g and offered greater sensitivity, specificity, and faster analysis without ethical constraints. Environmental parameters such as nutrients, water quality, and plankton community composition were also monitored to elucidate ecological drivers of toxin production. The findings represented that RBA was a reliable and efficient tool for PSP toxin monitoring, providing early-warning capability for HAB-related risks in tropical marine ecosystems.

Keywords


Harmful Algal Bloom (HAB) Radiolabeled Receptor Binding Assay (RBA) Saxitoxin Shellfish

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DOI: https://doi.org/10.55981/aij.2026.1710

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